Effects of Dexmedetomidine on the Expression of Cytochrome C Oxidase in Neonatal Mouse Primary Hippocampal Neurons Cultured in Propofol

To observe the effects of propofol and dexmedetomidine on expression cytochrome c oxidase in primary hippocampal neurons isolated from neonatal mice. C57 mice were sacrificed within 24 h birth tissue was collected. Primary cultured vitro then randomly divided into a control group (0 μM 0 dexmedetomidine), (20 dexmedetomidine) propofol+dexmedetomidine 10 dexmedetomidine). The incubated for 1 h. Neuron morphology observed under light microscope. protein detected semiquantitatively by Western blot immunofluorescence staining. Compared with those group, morphologically degenerated, sparse axons dendrites; significantly higher (p<0.0001). similar to that plump bright cell bodies thickened neurites intertwined neuron network; additionally, lower than Dexmedetomidine reversed induced injury morphological abnormalities neurons. mechanism may be related downregulation expression.